Influence of extraction solvent on the biological properties of maritime pine bark (Pinus pinaster)

Inés Mármol, Catarina Vieito, Vanessa Andreu, Annabel Levert, Anaïs Amiot, Cédric Bertrand, Mª Jesús Rodríguez-Yoldi, Joana Santos, Manuela Vaz-Velho


Maritime pine bark (Pinus pinaster Aiton subsp. atlantica) is rich in polyphenols with known bioactive properties which are beneficial for human health. However, biological activities of bark extracts depend on the type of polyphenols extracted and the characteristics of these extractives depend on several factors such as the type of solvents used. The influence of the extraction solvent on the composition and consequently on the properties of the extracts has been poorly described. Thus, in this study the influence of the extraction solvent (water, ethanol and ethanol-water (50/50 v/v%)) on the antibacterial and anticancer properties of P. pinaster bark samples were evaluated. LC-DAD-MS profiling of the different extracts was also carried out to study their polyphenol composition. Results show that extraction solvent must be carefully chosen with respect to foreseeing use of bark extracts, since ethanolic and hydroethanolic extracts displayed the greatest antibacterial activity whereas water extracts showed increased anticancer properties.


Pinus pinaster; pine bark; extraction solvent; LC-DAD-MS; antibacterial; anticancer

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